Application of flexible flask for static culture of adherent cells

Conventional T-flasks with different sizes and layers are commonly used devices for static cell culture at laboratory scale. Due to their non-deformable structure and requirement of strict manufacture art, the rigid T-flask costs highly in production and transportation and, of course, produces more waste. In this study, we described a pentahedron shaped flexible flask made of a medical application plastic film and reported its application in anchorage-dependent cell culture. The results showed that the flexible flasks achieved compatible efficiencies with T-flasks in the yield, viability and homogenous distribution of Vero cells and 293 cells. We concluded that the atactic polypropylene and styrene-ethylene-butene-styrene-copolymer plastic film was a good biocompatible material with cells and the novel flexible flask is a cost-effective alternative culture vessel for static culture of adherent cells at laboratory scale and possesses several special advantages such as simple to manufacture, foldable to save shipping and storage room and low in waste generation. [Zhang XL, Yang T, Han ZQ, Liu HT, Huang Y, Sun X, Mao CY, Xu YM. Application of flexible flask for static culture of adherent cells. Life Sci J 2014;11(2):419-423]. (ISSN:1097-8135). http://www.lifesciencesite.com. 57